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KMID : 0624620200530020094
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BMB Reports
2020 Volume.53 No. 2 p.94 ~ p.99
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Functional Analysis of RNA Motifs Essential for BC200 RNA-mediated Translational Regulation
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Jang Seong-Hui
Shin Hee-Gwon
Lee Young-Hoon
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Abstract
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Brain cytoplasmic 200 RNA (BC200 RNA) is proposed to act as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translational-inhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation.
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KEYWORD
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A/C-rich domain, Alu domain, BC200 RNA, RNA motifs, Translational regulation
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